单克隆抗体

FLAG(1E2)兔单克隆抗体

一般信息 / General Information

目录号	R17014ME2
克隆号	1E2
抗体类型	Rabbit IgG
实验应用	ELISA, WB, IHC, IF
推荐稀释比例	ELISA 1ug/ml WB 1ug/ml IF 1:100-1:200 FC 1:100-1:200
种属反应性	DYKDDDDK(same epitope to Sigma FLAG tag)
免疫原	DYKDDDDK-KLH conjugate
存储缓冲液	0.22 µM filtered solution of PBS,pH 7.4

背景 / Background

DYKDDDDK polypeptide tag (i.e. FLAG® tag, currently owned by Sigma) is a common protein tag added to recombinant proteins. DYKDDDDK was originally used by Munro and Pelham in 1984. This artificially designed tag facilitates the identification and purification of recombinant proteins. The hydrophilic nature of DYKDDDDK make it less likely to denature or inactivate proteins to which it is fused. Moreover, N-terminal FLAG tags can be readily cleaved from proteins with enterokinase. It is one of the most widely used protein tags.

图片 / Picture

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存储条件 / Storage

Shipped at 4°C. Store at +4°C short term (2-4 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

缩略语 / Abbreviations

ITA :Immuno Turbidimetric assay
WB: Western Blot
IF: immunofluorescence
IP: Immuno precipitation
ELISA: Enzyme-linked Immunosorbent assay
IHC: Immunohistochemistry
FC: flow cytometry

生物危害 / Hazard/Biohazard

This antibody contains 0.09% sodium azide as preserva-tive. Please handle and dispose the product properly. No known biohazard is associated with this product.

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